The new DNA trials off twenty-four inhabitants founders were used to make TruSeq Nextera sequencing libraries during the Genomics facility during the Cornell School. Products from all of the 24 founders have been pooled and you can sequenced into the an excellent single lane from dos of the 150 bp checks out into an enthusiastic Illumina NextSeq500 instrument resulting in typically 8x exposure per private. Samples throughout the degree place was in fact pooled in one way with 2,736 people and you will sequenced within 2 by the 150 bp reads on a keen Illumina NextSeq500 appliance, leading to whenever 0.1x coverage per individual. Genotyping-by-sequencing (GBS) study having assessment which have PHG genotypes was in fact out of Muleta et al. (unpublished data, 2019).
dos.cuatro Building the brand new sorghum PHG
Good sorghum simple haplotype chart try founded having fun with programs from the p_sorghumphg bitbucket repository and you can PHG version 0.0.nine.